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Cultured endothelial cells labeled with Anti-Nos-3/ENOSPolyclonal Antibody (bs-0163R), Unconjugated, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG,FITC conjugated (bs-0295G-FITC) at 1:200 dilution for 40 min.
Cultured endothelial cells labeled with Anti-Nos-3/ENOSPolyclonal Antibody (bs-0163R), Unconjugated, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG,FITC conjugated (bs-0295G-FITC) at 1:200 dilution for 40 min.
Cultured endothelial cells labeled with Anti-Nos-3/ENOSPolyclonal Antibody (bs-0163R), Unconjugated, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG,FITC conjugated (bs-0295G-FITC) at 1:200 dilution for 40 min.

eNOS Polyclonal Antibody

BS-0163R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNOS3
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    eNOS Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4846
  • Target name
    NOS3
  • Target description
    nitric oxide synthase 3
  • Target synonyms
    cNOS; constitutive NOS; ECNOS; EC-NOS; endothelial NOS; eNOS; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; nitric oxide synthase, endothelial; NOS type III; NOSIII
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29474
  • Protein Name
    Nitric oxide synthase, endothelial
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Compressive force-induced autophagy in periodontal ligament cells downregulates osteoclastogenesis during tooth movement. Chen L et al., 2019 Oct, J Periodontol
    Read more
  • Oxidative stress mediates renal endothelial cell damage in trichloroethylene-sensitized mice. Li B et al., 2019, J Toxicol Sci
    Read more
  • Ginsenoside Re inhibits vascular neointimal hyperplasia in balloon-injured carotid arteries through activating the eNOS/NO/cGMP pathway in rats. Gao Y et al., 2018 Oct, Biomed Pharmacother
    Read more
  • Neuropathy optic glaucomatosa induced by systemic hypertension through activation endothelin-1 signaling pathway in central retinal artery in rats. Prayitnaningsih S et al., 2016, Int J Ophthalmol
    Read more
  • In situ eNOS/NO up-regulation-a simple and effective therapeutic strategy for diabetic skin ulcer. Yang Y et al., 2016 Jul 25, Sci Rep
    Read more
  • Erythropoietin promotes retinal angiogenesis in a mouse model. Wen CT et al., 2014 Dec, Mol Med Rep
    Read more
  • Icariin, a phosphodiesterase-5 inhibitor, improves learning and memory in APP/PS1 transgenic mice by stimulation of NO/cGMP signalling. Jin F et al., 2014 Jun, Int J Neuropsychopharmacol
    Read more

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Figure 1. Western blot analysis of NOS3 using anti-NOS3 antibody (A01604-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates Lane 2: human K562 whole cell lysates Lane 3: human HepG2 whole cell lysates Lane 4: human THP-1 whole cell lysates Lane 5: rat kidney tissue lysates Lane 6: mouse kidne tissue lysates Lane 7: mouse small intestine tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOS3 antigen affinity purified polyclonal antibody (Catalog # A01604-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOS3 at approximately 133KD. The expected band size for NOS3 is at 133KD.
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