eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein at cell membrane by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: eNOS (phospho Ser1177) stained by eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein at cell membrane by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: eNOS (phospho Ser1177) stained by eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
eNOS (phospho Ser1177) antibody
GTX129058
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNOS3
Overview
- SupplierGeneTex
- Product NameeNOS (phospho Ser1177) antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.05 mg/ml
- ConjugateUnconjugated
- Gene ID4846
- Target nameNOS3
- Target descriptionnitric oxide synthase 3
- Target synonymsECNOS, eNOS, nitric oxide synthase 3, EC-NOS, NOS type III, NOSIII, cNOS, constitutive NOS, endothelial NOS, nitric oxide synthase 3 (endothelial cell)
- HostRabbit
- IsotypeIgG
- Protein IDP29474
- Protein NameNitric oxide synthase 3
- Scientific DescriptionNitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Chang TT, Chen C, Lin LY, et al. CCL4 Deletion Accelerates Wound Healing by Improving Endothelial Cell Functions in Diabetes Mellitus. Biomedicines. 2022,10(8). doi: 10.3390/biomedicines10081963Read this paper
- Fan Y, Fan H, Zhu B, et al. Astragaloside IV protects against diabetic nephropathy via activating eNOS in streptozotocin diabetes-induced rats. BMC Complement Altern Med. 2019,19(1):355. doi: 10.1186/s12906-019-2728-9Read this paper
- García-Arroyo FE, Monroy-Sánchez F, Muñoz-Jiménez I, et al. Allopurinol Prevents the Lipogenic Response Induced by an Acute Oral Fructose Challenge in Short-Term Fructose Fed Rats. Biomolecules. 2019,9(10). doi: 10.3390/biom9100601Read this paper
- Papinska AM, Rodgers KE. Long-Term Administration of Angiotensin (1-7) to db/db Mice Reduces Oxidative Stress Damage in the Kidneys and Prevents Renal Dysfunction. Oxid Med Cell Longev. 2018,2018:1841046. doi: 10.1155/2018/1841046Read this paper
- Yamada T, Adachi T, Ido Y, et al. Preserved Vasoconstriction and Relaxation of Saphenous Vein Grafts Obtained by a No-Touch Technique for Coronary Artery Bypass Grafting. Circ J. 2018,83(1):232-238. doi: 10.1253/circj.CJ-18-0714Read this paper
Datasheet
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![WB analysis of Bend3 cells using GTX42866 eNOS (phospho Ser1177) antibody [BDI250] at 1 μg/mL.
Lane 1 : Untreated whole cell lysate
Lane 2 : Pervanadate-treated whole cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX42866/GTX42866_19_WB_w_23060820_309.webp)
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![Untreated (–) and treated (+) HUVEC whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with eNOS (phospho Ser1177) antibody [HL3498] (GTX641386) diluted at 1:500 and eNOS antibody (GTX133404) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641386/GTX641386_T-45607_20250314_WB_treatment_Pervanadate_25031900_574.webp)
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