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eNOS (phospho Ser1177) antibody

GTX129058
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetNOS3
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Overview

  • Supplier
    GeneTex
  • Product Name
    eNOS (phospho Ser1177) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.05 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4846
  • Target name
    NOS3
  • Target description
    nitric oxide synthase 3
  • Target synonyms
    cNOS; constitutive NOS; ECNOS; EC-NOS; endothelial NOS; eNOS; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; nitric oxide synthase, endothelial; NOS type III; NOSIII
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29474
  • Protein Name
    Nitric oxide synthase, endothelial
  • Scientific Description
    Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • CCL4 Deletion Accelerates Wound Healing by Improving Endothelial Cell Functions in Diabetes Mellitus.
    Read more
  • Astragaloside IV protects against diabetic nephropathy via activating eNOS in streptozotocin diabetes-induced rats. Fan Y et al., 2019 Dec 5, BMC Complement Altern Med
    Read more
  • Allopurinol Prevents the Lipogenic Response Induced by an Acute Oral Fructose Challenge in Short-Term Fructose Fed Rats. Garcia-Arroyo FE et al., 2019 Oct 11, Biomolecules
    Read more
  • Long-Term Administration of Angiotensin (1-7) to db/db Mice Reduces Oxidative Stress Damage in the Kidneys and Prevents Renal Dysfunction. Papinska AM et al., 2018, Oxid Med Cell Longev
    Read more
  • Preserved Vasoconstriction and Relaxation of Saphenous Vein Grafts Obtained by a No-Touch Technique for Coronary Artery Bypass Grafting. Yamada T et al., 2018 Dec 25, Circ J
    Read more

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Figure 1. Western blot analysis of NOS3 using anti-NOS3 antibody (A01604-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates Lane 2: human K562 whole cell lysates Lane 3: human HepG2 whole cell lysates Lane 4: human THP-1 whole cell lysates Lane 5: rat kidney tissue lysates Lane 6: mouse kidne tissue lysates Lane 7: mouse small intestine tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOS3 antigen affinity purified polyclonal antibody (Catalog # A01604-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOS3 at approximately 133KD. The expected band size for NOS3 is at 133KD.
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