Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ENY2 antibody [GT245] (GTX629542) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
![Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with ENY2 antibody [GT245] (GTX629542) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX629542/GTX629542_41372_20201218_WB_1_w_23061202_716.webp "Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with ENY2 antibody [GT245] (GTX629542) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.")
![ENY2 antibody [GT245] detects ENY2 protein at nucleus in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. ENY2 antibody [GT245] (GTX629542) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX629542/GTX629542_41372_20160616_IHC-P_R_w_23061202_351.webp "ENY2 antibody [GT245] detects ENY2 protein at nucleus in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. ENY2 antibody [GT245] (GTX629542) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ENY2 antibody [GT245] (GTX629542) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
ENY2 antibody [GT245]
GTX629542
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetENY2
Overview
- SupplierGeneTex
- Product NameENY2 antibody [GT245]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT245
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID56943
- Target nameENY2
- Target descriptionENY2 transcription and export complex 2 subunit
- Target synonymsDC6, Sus1, e(y)2, transcription and mRNA export factor ENY2, enhancer of yellow 2 homolog, enhancer of yellow 2 transcription factor homolog
- HostMouse
- IsotypeIgG1
- Protein IDQ9NPA8
- Protein NameTranscription and mRNA export factor ENY2
- Scientific DescriptionComponent of the transcription regulatory histone acetylation (HAT) complex SAGA, a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates to a subcomplex that specifically deubiquitinates both histones H2A and H2B. The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation. May also participate in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery.
- ReactivityHuman, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Ng PK, Li J, Jeong KJ, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018,33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021Read this paper
Datasheet
Related products
Product group Antibodies
Anti-ENY2 (N-term) Antibody102-26826
ApplicationsWestern Blot
TargetENY2
- SizePrice
Product group Antibodies
ENY2 AntibodyCSB-PA889067LA01HU
ApplicationsELISA
ReactivityHuman
TargetENY2
- SizePrice
Product group Antibodies
ENY2 Antibody (FITC)LS-C380129
ApplicationsELISA
ReactivityHuman
TargetENY2
- SizePrice
Product group Antibodies
Anti-ENY2 AntibodyHPA024648
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetENY2
- SizePrice
Product group Antibodies
References
ENY2 antibodyGTX128034
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetENY2
- SizePrice