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Western Blot analysis of 293 cells using p300 Polyclonal Antibody
Western Blot analysis of 293 cells using p300 Polyclonal Antibody
Western Blot analysis of 293 cells using p300 Polyclonal Antibody

EP300 Antibody

CSB-PA003640
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetEP300
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Overview

  • Supplier
    Cusabio
  • Product Name
    EP300 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2033
  • Target name
    EP300
  • Target description
    EP300 lysine acetyltransferase
  • Target synonyms
    KAT3B, MKHK2, RSTS2, p300, histone acetyltransferase p300, E1A binding protein p300, E1A-associated protein p300, E1A-binding protein, 300kD, histone butyryltransferase p300, histone crotonyltransferase p300, p300 HAT, protein 2-hydroxyisobutyryltransferase p300, protein lactyltransferas p300, protein propionyltransferase p300
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ09472
  • Protein Name
    Histone acetyltransferase p300
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KAT3B/P300/EP300 using anti-KAT3B/P300/EP300 antibody (A00117). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAT3B/P300/EP300 antigen affinity purified polyclonal antibody (Catalog # A00117) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAT3B/P300/EP300 at approximately 264-300 kDa. The expected band size for KAT3B/P300/EP300 is at 264 kDa.
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