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WB analysis of various sample lysates using GTX54723 Epac1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX54723 Epac1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX54723 Epac1 antibody. Dilution : 1:1000 Loading : 25microg per lane

Epac1 antibody

GTX54723
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetRAPGEF3
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Overview

  • Supplier
    GeneTex
  • Product Name
    Epac1 antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10411
  • Target name
    RAPGEF3
  • Target description
    Rap guanine nucleotide exchange factor 3
  • Target synonyms
    CAMP-GEFI, EPAC, EPAC1, HSU79275, bcm910, rap guanine nucleotide exchange factor 3, 9330170P05Rik, EPAC 1, Rap guanine nucleotide exchange factor (GEF) 3, Rap1 guanine-nucleotide-exchange factor directly activated by cAMP, cAMP-regulated guanine nucleotide exchange factor I, exchange factor directly activated by cAMP 1, exchange protein directly activated by cAMP 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95398
  • Protein Name
    Rap guanine nucleotide exchange factor 3
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of Epac1/RAPGEF3 using anti-Epac1/RAPGEF3 antibody (A02483-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: monkey kidney tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Epac1/RAPGEF3 antigen affinity purified polyclonal antibody (Catalog # A02483-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Epac1/RAPGEF3 at approximately 104KD. The expected band size for Epac1/RAPGEF3 is at 104KD.
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