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ProductsBack/EpCAM antibody [VU-1D9]
IHC-P analysis of human breast carcinoma tissue using GTX28667 EpCAM antibody [VU-1D9].
IHC-P analysis of human breast carcinoma tissue using GTX28667 EpCAM antibody [VU-1D9].
IHC-P analysis of human breast carcinoma tissue using GTX28667 EpCAM antibody [VU-1D9].

EpCAM antibody [VU-1D9]

GTX28667
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetEPCAM
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Overview

  • Supplier
    GeneTex
  • Product Name
    EpCAM antibody [VU-1D9]
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1-2 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    VU-1D9
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID4072
  • Target name
    EPCAM
  • Target description
    epithelial cell adhesion molecule
  • Target synonyms
    adenocarcinoma-associated antigen; cell surface glycoprotein Trop-1; DIAR5; EGP-2; EGP314; EGP40; epithelial cell adhesion molecule; epithelial glycoprotein 314; ESA; HNPCC8; human epithelial glycoprotein-2; KS1/4; KSA; M4S1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); MIC18; MK-1; TACSTD1; TROP1; trophoblast cell surface antigen 1; tumor-associated calcium signal transducer 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP16422
  • Protein Name
    Epithelial cell adhesion molecule
  • Scientific Description
    This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Transcriptional changes in the mammary gland during lactation revealed by single cell sequencing of cells from human milk. Twigger AJ et al., 2022 Jan 28, Nat Commun
    Read more
  • Virtual-freezing fluorescence imaging flow cytometry. Mikami H et al., 2020 Mar 6, Nat Commun
    Read more
  • Preferential capture of EpCAM-expressing extracellular vesicles on solid surfaces coated with an aptamer-conjugated zwitterionic polymer.
    Read more
  • Optimisation of immunofluorescence methods to determine MCT1 and MCT4 expression in circulating tumour cells. Kershaw S et al., 2015 May 10, BMC Cancer
    Read more
  • Mutational analysis of circulating tumor cells from colorectal cancer patients and correlation with primary tumor tissue. Lyberopoulou A et al., 2015, PLoS One
    Read more
  • Superficial scrapings from breast tumors is a source for biobanking and research purposes. Ma R et al., 2014 Jul, Lab Invest
    Read more
  • High-purity separation of cancer cells by optically induced dielectrophoresis. Chen HH et al., 2014 Apr, J Biomed Opt
    Read more
  • Hyaluronan synthase HAS2 promotes tumor progression in bone by stimulating the interaction of breast cancer stem-like cells with macrophages and stromal cells. Okuda H et al., 2012 Jan 15, Cancer Res
    Read more
  • Characterization of the epithelial cell adhesion molecule (EpCAM)+ cell population in hepatocellular carcinoma cell lines. Kimura O et al., 2010 Oct, Cancer Sci
    Read more

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Figure 1. Western blot analysis of EPCAM using anti-EPCAM antibody (PB10059). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPCAM antigen affinity purified polyclonal antibody (Catalog # PB10059) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPCAM at approximately 35-40 kDa. The expected band size for EPCAM is at 35 kDa.
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