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Western Blot analysis of 293T cells using Epigen Polyclonal Antibody
Western Blot analysis of 293T cells using Epigen Polyclonal Antibody
Western Blot analysis of 293T cells using Epigen Polyclonal Antibody

EPGN Antibody

CSB-PA006336
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetEPGN
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Overview

  • Supplier
    Cusabio
  • Product Name
    EPGN Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID255324
  • Target name
    EPGN
  • Target description
    epithelial mitogen
  • Target synonyms
    ALGV3072, EPG, PRO9904, epigen, epithelial mitogen homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6UW88
  • Protein Name
    Epigen
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Epigen using anti-Epigen antibody (A10011-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole Cell lysate, Lane 2: rat PC-12 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Epigen antigen affinity purified polyclonal antibody (Catalog # A10011-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Epigen at approximately 17KD. The expected band size for Epigen is at 17KD.
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