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Western Blot analysis of COLO205 cells using EphA1 Polyclonal Antibody
Western Blot analysis of COLO205 cells using EphA1 Polyclonal Antibody
Western Blot analysis of COLO205 cells using EphA1 Polyclonal Antibody

EPHA1 Antibody

CSB-PA002360
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetEPHA1
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Overview

  • Supplier
    Cusabio
  • Product Name
    EPHA1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2041
  • Target name
    EPHA1
  • Target description
    EPH receptor A1
  • Target synonyms
    EPH, EPHT, EPHT1, ephrin type-A receptor 1, eph tyrosine kinase 1, erythropoietin-producing hepatoma amplified sequence, erythropoietin-producing hepatoma receptor, hEpha1, oncogene EPH, tyrosine-protein kinase receptor EPH
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21709
  • Protein Name
    Ephrin type-A receptor 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA1 antibody [C1C3-2] (GTX111449) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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Figure 1. Western blot analysis of Eph receptor A1 using anti-Eph receptor A1 antibody (PA1573). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Colo320 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eph receptor A1 antigen affinity purified polyclonal antibody (Catalog # PA1573) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eph receptor A1 at approximately 113 kDa. The expected band size for Eph receptor A1 is at 108 kDa.
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