Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA4 antibody [N3C2-2], Internal (GTX111602) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA4 antibody [N3C2], Internal (GTX111602) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111602/GTX111602_40618_20170608_WB_M_hippocampus_w_23060500_667.webp "Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA4 antibody [N3C2], Internal (GTX111602) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Eph receptor A4 antibody [N3C2-2], Internal detects Eph receptor A4 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5 % SDS-PAGE, and blotted with Eph receptor A4 antibody [N3C2-2], Internal (GTX111602) diluted by 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX111602/GTX111602_40618_WB_2_w_23060500_742.webp "Eph receptor A4 antibody [N3C2-2], Internal detects Eph receptor A4 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5 % SDS-PAGE, and blotted with Eph receptor A4 antibody [N3C2-2], Internal (GTX111602) diluted by 1:1000")
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA4 antibody [N3C2-2], Internal (GTX111602) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
EphA4 antibody [N3C2-2], Internal
GTX111602
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetEPHA4
Overview
- SupplierGeneTex
- Product NameEphA4 antibody [N3C2-2], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.97 mg/ml
- ConjugateUnconjugated
- Gene ID2043
- Target nameEPHA4
- Target descriptionEPH receptor A4
- Target synonymsEK8, HEK8, SEK, TYRO1, ephrin type-A receptor 4, EPH-like kinase 8, TYRO1 protein tyrosine kinase, receptor protein-tyrosine kinase HEK8, tyrosine-protein kinase TYRO1, tyrosine-protein kinase receptor SEK
- HostRabbit
- IsotypeIgG
- Protein IDP54764
- Protein NameEphrin type-A receptor 4
- Scientific DescriptionThis gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Li Z, Yu Y, Kang J, et al. MicroRNA-124 Overexpression in Schwann Cells Promotes Schwann Cell-Astrocyte Integration and Inhibits Glial Scar Formation Ability. Front Cell Neurosci. 2020,14:144. doi: 10.3389/fncel.2020.00144Read this paper
- Hua KT, Hong JB, Sheen YS, et al. miR-519d Promotes Melanoma Progression by Downregulating EphA4. Cancer Res. 2018,78(1):216-229. doi: 10.1158/0008-5472.CAN-17-1933Read this paper
Datasheet
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