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Lane 1: U-251 MG Cell lysates; probed with EPHA5 (46CT61.6.4) Monoclonal Antibody, unconjugated (bsm-51268M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: U-251 MG Cell lysates; probed with EPHA5 (46CT61.6.4) Monoclonal Antibody, unconjugated (bsm-51268M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: U-251 MG Cell lysates; probed with EPHA5 (46CT61.6.4) Monoclonal Antibody, unconjugated (bsm-51268M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

EPHA5 (6C4) Monoclonal Antibody

BSM-51268M
Bioss
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetEPHA5
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Overview

  • Supplier
    Bioss
  • Product Name
    EPHA5 (6C4) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6C4
  • Concentration
    0.5 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID2044
  • Target name
    EPHA5
  • Target description
    EPH receptor A5
  • Target synonyms
    CEK7, EHK-1, EHK1, EK7, HEK7, TYRO4, ephrin type-A receptor 5, EPH homology kinase 1, EPH-like kinase 7, brain-specific kinase, epididymis secretory sperm binding protein, receptor protein-tyrosine kinase HEK7, tyrosine-protein kinase receptor EHK-1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP54756
  • Protein Name
    Ephrin type-A receptor 5
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Eph receptor A5 using anti-Eph receptor A5 antibody (PB9583). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug Lane 2: Mouse Brain Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eph receptor A5 antigen affinity purified polyclonal antibody (Catalog # PB9583) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eph receptor A5 at approximately 114 kDa. The expected band size for Eph receptor A5 is at 114 kDa.
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