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Western Blot analysis of A549 cells using EphA5 Polyclonal Antibody
Western Blot analysis of A549 cells using EphA5 Polyclonal Antibody
Western Blot analysis of A549 cells using EphA5 Polyclonal Antibody

EPHA5 Antibody

CSB-PA002363
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetEPHA5
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Overview

  • Supplier
    Cusabio
  • Product Name
    EPHA5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2044
  • Target name
    EPHA5
  • Target description
    EPH receptor A5
  • Target synonyms
    CEK7, EHK-1, EHK1, EK7, HEK7, TYRO4, ephrin type-A receptor 5, EPH homology kinase 1, EPH-like kinase 7, brain-specific kinase, epididymis secretory sperm binding protein, receptor protein-tyrosine kinase HEK7, tyrosine-protein kinase receptor EHK-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP54756
  • Protein Name
    Ephrin type-A receptor 5
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Eph receptor A5 using anti-Eph receptor A5 antibody (PB9583). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug Lane 2: Mouse Brain Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eph receptor A5 antigen affinity purified polyclonal antibody (Catalog # PB9583) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eph receptor A5 at approximately 114 kDa. The expected band size for Eph receptor A5 is at 114 kDa.
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