Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-EphB2 R Polyclonal Antibody, Unconjugated (bs-0247R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining
EphB2 R Polyclonal Antibody
BS-0247R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Chicken, Human, Mouse, Rat
TargetEPHB2
Overview
- SupplierBioss Antibodies
- Product NameEphB2 R Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID2048
- Target nameEPHB2
- Target descriptionEPH receptor B2
- Target synonymsBDPLT22; CAPB; developmentally-regulated Eph-related tyrosine kinase; DRT; EK5; elk-related tyrosine kinase; eph tyrosine kinase 3; EPH-like kinase 5; ephrin type-B receptor 2; EPHT3; ERK; Hek5; PCBC; protein-tyrosine kinase HEK5; renal carcinoma antigen NY-REN-47; Tyro5; tyrosine-protein kinase receptor EPH-3; tyrosine-protein kinase TYRO5
- HostRabbit
- IsotypeIgG
- Protein IDP29323
- Protein NameEphrin type-B receptor 2
- ReactivityCanine, Chicken, Human, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203