Escherichia coli expressed recombinant human EPO protein were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (GTX637446) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
![Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human EPO protein, His and GST tag (active) (GTX00422-pro) (500-7.81 ng/mL). Coated protein was probed with EPO antibody [HL1794] (GTX637446) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637446/GTX637446_44858_20221111_ELISA_Indirect_22112219_456.webp "Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human EPO protein, His and GST tag (active) (GTX00422-pro) (500-7.81 ng/mL). Coated protein was probed with EPO antibody [HL1794] (GTX637446) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.")
![Indirect ELISA analysis was performed by coating the plate with recombinant CHO cells expressed, full-length human EPO protein (500-7.81 ng/mL). Coated protein was probed with EPO antibody [HL1794] (GTX637446) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637446/GTX637446_44858_20221202_ELISA_Indirect_22121123_203.webp "Indirect ELISA analysis was performed by coating the plate with recombinant CHO cells expressed, full-length human EPO protein (500-7.81 ng/mL). Coated protein was probed with EPO antibody [HL1794] (GTX637446) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.")
![Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below. Capture: EPO antibody [HL1794] (GTX637446) (5 μg/mL) Detection: HRP-conjugated EPO antibody [HL1793] (GTX637445) (1 μg/mL) Please notice that GTX637445 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637446. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637446/GTX637446_44858_20221223_ELISA_PAIR_22122722_809.webp "Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below. Capture: EPO antibody [HL1794] (GTX637446) (5 μg/mL) Detection: HRP-conjugated EPO antibody [HL1793] (GTX637445) (1 μg/mL) Please notice that GTX637445 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637446. Please contact us for custom HRP-conjugated antibody.")
![Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below. Capture: EPO antibody [HL1793] (GTX637445) (5 μg/mL) Detection: HRP-conjugated EPO antibody [HL1794] (GTX637446) (1 μg/mL) Please notice that GTX637446 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637445. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637446/GTX637446_44858_20221223_ELISA_PAIR_2_22122722_709.webp "Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below. Capture: EPO antibody [HL1793] (GTX637445) (5 μg/mL) Detection: HRP-conjugated EPO antibody [HL1794] (GTX637446) (1 μg/mL) Please notice that GTX637446 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637445. Please contact us for custom HRP-conjugated antibody.")
![Recombinant human EPO protein (CHO cells expressed) was separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (GTX637446) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637446/GTX637446_44858_20221223_WB_Ag_22122722_430.webp "Recombinant human EPO protein (CHO cells expressed) was separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (GTX637446) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![HepG2 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (GTX637446) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637446/GTX637446_44858_20221223_WB_Fraction_22122722_234.webp "HepG2 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (GTX637446) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![EPO antibody [HL1794] detects secreted EPO protein by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. EPO stained by EPO antibody [HL1794] (GTX637446) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637446/GTX637446_44858_20230217_IHC-P_Cat_23021401_577.webp "EPO antibody [HL1794] detects secreted EPO protein by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. EPO stained by EPO antibody [HL1794] (GTX637446) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![EPO antibody [HL1794] detects secreted EPO protein by immunohistochemical analysis. Sample: Paraffin-embedded dog kidney. EPO stained by EPO antibody [HL1794] (GTX637446) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637446/GTX637446_44858_20230217_IHC-P_Dog_23021401_553.webp "EPO antibody [HL1794] detects secreted EPO protein by immunohistochemical analysis. Sample: Paraffin-embedded dog kidney. EPO stained by EPO antibody [HL1794] (GTX637446) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![The cell proliferation of the TF-1 human erythroleukemic cell line induced by Human Erythropoietin/EPO and neutralization by Erythropoietin Antibody. Proliferation elicited by Recombinant Human Erythropoietin/EPO (0.5 ng/mL) is neutralized by increasing concentrations of EPO antibody [HL1794] (GTX637446).](https://www.genetex.com/upload/website/prouct_img/normal/GTX637446/GTX637446_44858_20240524_Functionalassay_24052802_246.webp "The cell proliferation of the TF-1 human erythroleukemic cell line induced by Human Erythropoietin/EPO and neutralization by Erythropoietin Antibody. Proliferation elicited by Recombinant Human Erythropoietin/EPO (0.5 ng/mL) is neutralized by increasing concentrations of EPO antibody [HL1794] (GTX637446).")
Escherichia coli expressed recombinant human EPO protein were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (GTX637446) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
EPO antibody [HL1794]
GTX637446
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
ReactivityCanine, Feline, Human
TargetEPO
Overview
- SupplierGeneTex
- Product NameEPO antibody [HL1794]
- Delivery Days Customer9
- Application Supplier Note*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1794
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2056
- Target nameEPO
- Target descriptionerythropoietin
- Target synonymsDBAL, ECYT5, EP, MVCD2, erythropoietin, epoetin
- HostRabbit
- IsotypeIgG
- Protein IDP01588
- Protein NameErythropoietin
- Scientific DescriptionThis gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies. [provided by RefSeq, Aug 2017]
- ReactivityCanine, Feline, Human
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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![The cell proliferation of the TF-1 human erythroleukemic cell line induced by Human Erythropoietin/EPO and neutralization by Erythropoietin Antibody. Proliferation elicited by Recombinant Human Erythropoietin/EPO (0.4 ng/mL) is neutralized by increasing concentrations of EPO antibody [HL2752] (GTX639615).](https://www.genetex.com/upload/website/prouct_img/normal/GTX639615/GTX639615_T-45278_20240524_Functionalassay_24052802_265.webp)
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