Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with EPO Polyclonal Antibody, Unconjugated (bs-2343R ) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with EPO Polyclonal Antibody, Unconjugated (bs-2343R ) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
EPO Polyclonal Antibody
BS-2343R
ApplicationsDot Blot, Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetEPO
Overview
- SupplierBioss Antibodies
- Product NameEPO Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsDot Blot, Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200), dot-blot()
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID2056
- Target nameEPO
- Target descriptionerythropoietin
- Target synonymsDBAL; ECYT5; EP; epoetin; erythropoietin; MVCD2
- HostRabbit
- IsotypeIgG
- Protein IDP01588
- Protein NameErythropoietin
- Storage Instruction-20°C
- UNSPSC12352203
References
- Intranasal Erythropoietin Protects CA1 Hippocampal Cells, Modulated by Specific Time Pattern Molecular Changes After Ischemic Damage in Rats. Macias-Velez RJ et al., 2019 Aug, J Mol NeurosciRead more
- Detection of erythropoiesis stimulating agents in urine samples using a capillary Western system. Desharnais P et al., 2018 Nov, Drug Test AnalRead more
- Oxidative stress induces the decline of brain EPO expression in aging rats. Li X et al., 2016 Oct, Exp GerontolRead more
- Female cotton rats (Sigmodon hispidus) develop chronic anemia with renal inflammation and cystic changes. Ichii O et al., 2016 Sep, Histochem Cell BiolRead more
Datasheet
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