Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with EPO Polyclonal Antibody, Unconjugated (bs-2343R ) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with EPO Polyclonal Antibody, Unconjugated (bs-2343R ) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
EPO Polyclonal Antibody
BS-2343R
ApplicationsDot Blot, Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetEPO
Overview
- SupplierBioss
- Product NameEPO Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsDot Blot, Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200), dot-blot()
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID2056
- Target nameEPO
- Target descriptionerythropoietin
- Target synonymsDBAL, ECYT5, EP, MVCD2, erythropoietin, epoetin
- HostRabbit
- IsotypeIgG
- Protein IDP01588
- Protein NameErythropoietin
- Storage Instruction-20°C
- UNSPSC12352203
References
- Macias-Velez RJ, Fukushima-Díaz de León L, Beas-Zárate C, et al. Intranasal Erythropoietin Protects CA1 Hippocampal Cells, Modulated by Specific Time Pattern Molecular Changes After Ischemic Damage in Rats. J Mol Neurosci. 2019,68(4):590-602. doi: 10.1007/s12031-019-01308-wRead this paper
- Desharnais P, Naud JF, Ayotte C. Detection of erythropoiesis stimulating agents in urine samples using a capillary Western system. Drug Test Anal. 2018,10(11-12):1698-1707. doi: 10.1002/dta.2528Read this paper
- Li X, Chen Y, Shao S, et al. Oxidative stress induces the decline of brain EPO expression in aging rats. Exp Gerontol. 2016,83:89-93. doi: 10.1016/j.exger.2016.07.012Read this paper
- Ichii O, Nakamura T, Irie T, et al. Female cotton rats (Sigmodon hispidus) develop chronic anemia with renal inflammation and cystic changes. Histochem Cell Biol. 2016,146(3):351-62. doi: 10.1007/s00418-016-1438-3Read this paper
Datasheet
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