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EPOR Antibody

CAB2917
Assay Genie
TargetEPOR
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    EPOR Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID2057
  • Target name
    EPOR
  • Target description
    erythropoietin receptor
  • Target synonyms
    EPO-R, erythropoietin receptor
  • Host
    Rabbit
  • Protein IDP19235
  • Protein Name
    Erythropoietin receptor
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: 293T whole cell lysate, Hela whole cell lysate All lanes: EPOR antibody at 4.3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56, 27, 36 kDa Observed band size: 56 kDa
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WB analysis of A498 (A), pervanadate-treated MCF7 (B), NIH3T3 (C) whole cell lysates using GTX32177 EPO receptor (phospho Tyr426) antibody.
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Figure 1. Western blot analysis of EPO Receptor using anti-EPO Receptor antibody (A00427-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPO Receptor antigen affinity purified polyclonal antibody (Catalog # A00427-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPO Receptor at approximately 55KD. The expected band size for EPO Receptor is at 55KD.
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