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Western Blot Positive WB detected in: 293T whole cell lysate All lanes: EPRS antibody at 7.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 171 kDa Observed band size: 171 kDa
Western Blot Positive WB detected in: 293T whole cell lysate All lanes: EPRS antibody at 7.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 171 kDa Observed band size: 171 kDa
Western Blot Positive WB detected in: 293T whole cell lysate All lanes: EPRS antibody at 7.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 171 kDa Observed band size: 171 kDa

EPRS Antibody

CSB-PA007747LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetEPRS1
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Overview

  • Supplier
    Cusabio
  • Product Name
    EPRS Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2058
  • Target name
    EPRS1
  • Target description
    glutamyl-prolyl-tRNA synthetase 1
  • Target synonyms
    EARS, EPRS, GLUPRORS, HLD15, PARS, PIG32, QARS, QPRS, bifunctional glutamate/proline--tRNA ligase, bifunctional aminoacyl-tRNA synthetase, cell proliferation-inducing gene 32 protein, glutamate tRNA ligase, glutamatyl-prolyl-tRNA synthetase, glutaminyl-tRNA synthetase, proliferation-inducing gene 32 protein, proliferation-inducing protein 32, proline-tRNA ligase, prolyl-tRNA synthetase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07814
  • Protein Name
    Bifunctional glutamate/proline--tRNA ligase
  • Scientific Description
    Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the multisynthetase complex and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EPRS1/PARS using anti-EPRS1/PARS antibody (A02967-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hek293 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat pancreas tissue lysates, Lane 5: mouse pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPRS1/PARS antigen affinity purified polyclonal antibody (Catalog # A02967-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPRS1/PARS at approximately 170-180KD. The expected band size for EPRS1/PARS is at 171KD.
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