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ProductsBack/QARS/Glutamyl Prolyl tRNA synthetase/ProRS Polyclonal Antibody
Formalin-fixed and paraffin embedded rat gastric tissue labeled with Anti-Glutamyl Prolyl tRNA synthetase/ProRS Polyclonal Antibody, Unconjugated (bs-13393R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat gastric tissue labeled with Anti-Glutamyl Prolyl tRNA synthetase/ProRS Polyclonal Antibody, Unconjugated (bs-13393R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat gastric tissue labeled with Anti-Glutamyl Prolyl tRNA synthetase/ProRS Polyclonal Antibody, Unconjugated (bs-13393R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

QARS/Glutamyl Prolyl tRNA synthetase/ProRS Polyclonal Antibody

BS-13393R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetEPRS1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Glutamyl Prolyl tRNA synthetase Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2058
  • Target name
    EPRS1
  • Target description
    glutamyl-prolyl-tRNA synthetase 1
  • Target synonyms
    bifunctional aminoacyl-tRNA synthetase; bifunctional glutamate/proline--tRNA ligase; cell proliferation-inducing gene 32 protein; EARS; EPRS; GLUPRORS; glutamate tRNA ligase; glutamatyl-prolyl-tRNA synthetase; glutaminyl-tRNA synthetase; HLD15; PARS; PIG32; proliferation-inducing gene 32 protein; proliferation-inducing protein 32; proline-tRNA ligase; prolyl-tRNA synthetase; QARS; QPRS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07814
  • Protein Name
    Bifunctional glutamate/proline--tRNA ligase
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EPRS1/PARS using anti-EPRS1/PARS antibody (A02967-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hek293 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat pancreas tissue lysates, Lane 5: mouse pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPRS1/PARS antigen affinity purified polyclonal antibody (Catalog # A02967-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPRS1/PARS at approximately 170-180KD. The expected band size for EPRS1/PARS is at 171KD.
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