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ProductsBack/ERAB (HSD17B10) Mouse Monoclonal Antibody [Clone ID: OTI5E2]
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells(WT) and HSD17B10-Knockdown HeLa cells(KD) were separated by SDS-PAGE and immunoblotted with anti-HSD17B10 monoclonal antibody TA500813(1:2000).Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells(WT) and HSD17B10-Knockdown HeLa cells(KD) were separated by SDS-PAGE and immunoblotted with anti-HSD17B10 monoclonal antibody TA500813(1:2000).Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells(WT) and HSD17B10-Knockdown HeLa cells(KD) were separated by SDS-PAGE and immunoblotted with anti-HSD17B10 monoclonal antibody TA500813(1:2000).Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.

ERAB (HSD17B10) Mouse Monoclonal Antibody [Clone ID: OTI5E2]

CF500813
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetHSD17B10
Price on request
100 ug
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More than 130 suppliers
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Overview

  • Supplier
    OriGene
  • Product Name
    ERAB (HSD17B10) Mouse Monoclonal Antibody [Clone ID: OTI5E2]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5E2
  • Gene ID3028
  • Target name
    HSD17B10
  • Target description
    hydroxysteroid 17-beta dehydrogenase 10
  • Target synonyms
    17b-HSD10, ABAD, CAMR, DUPXp11.22, ERAB, HADH2, HCD2, HSD10MD, MHBD, MRPP2, MRX17, MRX31, MRXS10, SCHAD, SDR5C1, 3-hydroxyacyl-CoA dehydrogenase type-2, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, AB-binding alcohol dehydrogenase, amyloid-beta peptide binding alcohol dehydrogenase, endoplasmic reticulum-associated amyloid beta-peptide-binding protein, mitochondrial RNase P subunit 2, mitochondrial ribonuclease P protein 2, short chain L-3-hydroxyacyl-CoA dehydrogenase type 2, short chain type dehydrogenase/reductase XH98G2
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ99714
  • Protein Name
    3-hydroxyacyl-CoA dehydrogenase type-2
  • Scientific Description
    Carrier-free (BSA/glycerol-free) HSD17B10 mouse monoclonal antibody, clone OTI5E2 (formerly 5E2)
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of ERAB using anti-ERAB antibody (PB9336). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MOLT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERAB antigen affinity purified polyclonal antibody (Catalog # PB9336) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERAB at approximately 25 kDa. The expected band size for ERAB is at 27-30 kDa.
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