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ProductsBack/ErbB 4 (ERBB4) (1230-1250) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6C5]
Detection of endogenous erbB4: Whole cell extracts of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab erbB4-6C5 (0.5 ug/ ml) for 1h at 15-22°C and developed by ECL (exp. time: 30 sec). Lane 1: MDA-MB 231 Lane 2: MDA-MB 468 Lane 3: MCF-7 Lane 4: T47D Lane 5: SW480 Lane 6: SW620
Detection of endogenous erbB4: Whole cell extracts of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab erbB4-6C5 (0.5 ug/ ml) for 1h at 15-22°C and developed by ECL (exp. time: 30 sec). Lane 1: MDA-MB 231 Lane 2: MDA-MB 468 Lane 3: MCF-7 Lane 4: T47D Lane 5: SW480 Lane 6: SW620
Detection of endogenous erbB4: Whole cell extracts of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab erbB4-6C5 (0.5 ug/ ml) for 1h at 15-22°C and developed by ECL (exp. time: 30 sec). Lane 1: MDA-MB 231 Lane 2: MDA-MB 468 Lane 3: MCF-7 Lane 4: T47D Lane 5: SW480 Lane 6: SW620

ErbB 4 (ERBB4) (1230-1250) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6C5]

AM00056PU-N
OriGene
ApplicationsWestern Blot
DownloadMSDS
Product group Antibodies
TargetERBB4
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    ErbB 4 (ERBB4) (1230-1250) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6C5]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6C5
  • Gene ID2066
  • Target name
    ERBB4
  • Target description
    erb-b2 receptor tyrosine kinase 4
  • Target synonyms
    ALS19; avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; HER4; human epidermal growth factor receptor 4; p180erbB4; proto-oncogene-like protein c-ErbB-4; receptor tyrosine-protein kinase erbB-4; tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ15303
  • Protein Name
    Receptor tyrosine-protein kinase erbB-4
  • Scientific Description
    ErbB 4 (ERBB4) (1230-1250) (incl. pos. control) mouse monoclonal antibody, clone 6C5, Purified
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ERBB4 using anti-ERBB4 antibody (A00296-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERBB4 antigen affinity purified polyclonal antibody (Catalog # A00296-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERBB4 at approximately 180 kDa. The expected band size for ERBB4 is at 147 kDa.
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