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Western Blot analysis of Hela cell using ERBB4 Polyclonal Antibody at dilution of 1:200
Western Blot analysis of Hela cell using ERBB4 Polyclonal Antibody at dilution of 1:200
Western Blot analysis of Hela cell using ERBB4 Polyclonal Antibody at dilution of 1:200

ERBB4 Polyclonal Antibody

E-AB-10231
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    ERBB4 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.3mg/ml
  • Scientific Description
    This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ERBB4 using anti-ERBB4 antibody (A00296-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERBB4 antigen affinity purified polyclonal antibody (Catalog # A00296-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERBB4 at approximately 180 kDa. The expected band size for ERBB4 is at 147 kDa.
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