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ICC/IF analysis of COS7 cells transiently transfected with ERCC1 plasmid using GTX84555 ERCC1 antibody [2A9].
ICC/IF analysis of COS7 cells transiently transfected with ERCC1 plasmid using GTX84555 ERCC1 antibody [2A9].
ICC/IF analysis of COS7 cells transiently transfected with ERCC1 plasmid using GTX84555 ERCC1 antibody [2A9].

ERCC1 antibody [2A9]

GTX84555
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetERCC1
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Overview

  • Supplier
    GeneTex
  • Product Name
    ERCC1 antibody [2A9] - Overexpression Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:2000. ICC/IF: 1:100. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2A9
  • Concentration
    0.96 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2067
  • Target name
    ERCC1
  • Target description
    ERCC excision repair 1, endonuclease non-catalytic subunit
  • Target synonyms
    COFS4; DNA excision repair protein ERCC-1; excision repair cross-complementation group 1; excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence); RAD10; UV20
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP07992
  • Protein Name
    DNA excision repair protein ERCC-1
  • Scientific Description
    Structure-specific DNA repair endonuclease responsible for the 5-incision during DNA repair.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ERCC1 using anti-ERCC1 antibody (A00388-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat stomach tissue lysates, Lane 2: mouse stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERCC1 antigen affinity purified polyclonal antibody (Catalog # A00388-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERCC1 at approximately 33KD. The expected band size for ERCC1 is at 33KD.
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