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ProductsBack/CSB Polyclonal Antibody
Formalin-fixed and paraffin embedded rat kidney tissue labeled with Rabbit Anti-ERCC6 Polyclonal Antibody, Unconjugated (bs-14082R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat kidney tissue labeled with Rabbit Anti-ERCC6 Polyclonal Antibody, Unconjugated (bs-14082R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat kidney tissue labeled with Rabbit Anti-ERCC6 Polyclonal Antibody, Unconjugated (bs-14082R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

CSB Polyclonal Antibody

BS-14082R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetERCC6
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Overview

  • Supplier
    Bioss
  • Product Name
    CSB Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2074
  • Target name
    ERCC6
  • Target description
    ERCC excision repair 6, chromatin remodeling factor
  • Target synonyms
    ARMD5, CKN2, COFS, COFS1, CSB, CSB-PGBD3, POF11, RAD26, UVSS1, DNA excision repair protein ERCC-6, ERCC6-PGBD3 fusion protein, ATP-dependent helicase ERCC6, Chimeric CSB-PGBD3 protein, Chimeric ERCC6-PGBD3 protein, Cockayne syndrome group B protein, cockayne syndrome protein CSB, excision repair cross-complementation group 6, excision repair cross-complementing rodent repair deficiency, complementation group 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ03468
  • Protein Name
    DNA excision repair protein ERCC-6
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of CSB using anti-CSB antibody (PB10060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Siha whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSB antigen affinity purified polyclonal antibody (Catalog # PB10060) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSB at approximately 170 kDa. The expected band size for CSB is at 168 kDa.
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