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ICC/IF analysis of A549 cells using GTX15780 ERK1 antibody [12D11]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature
ICC/IF analysis of A549 cells using GTX15780 ERK1 antibody [12D11]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature
ICC/IF analysis of A549 cells using GTX15780 ERK1 antibody [12D11]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

ERK1 antibody [12D11]

GTX15780
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Primate
TargetMAPK3
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Overview

  • Supplier
    GeneTex
  • Product Name
    ERK1 antibody [12D11]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. ICC/IF: 2 microg/ml. IHC-P: 1:100. ChIP assay: 1-3 microl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    12D11
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5595
  • Target name
    MAPK3
  • Target description
    mitogen-activated protein kinase 3
  • Target synonyms
    ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK, mitogen-activated protein kinase 3, MAPK 1, extracellular signal-regulated kinase 1, extracellular signal-related kinase 1, insulin-stimulated MAP2 kinase, microtubule-associated protein 2 kinase
  • Host
    Mouse
  • Isotype
    IgG
  • Protein IDP27361
  • Protein Name
    Mitogen-activated protein kinase 3
  • Scientific Description
    The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Mouse, Primate
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161