IHC-P analysis of human colon tissue using GTX15780 ERK1 antibody [12D11]. Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes at 95oC
![WB analysis of 25 ug of various whole cell lysates using GTX15780 ERK1 antibody [12D11]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX15780/GTX15780_1534_WB_w_23060620_378.webp "WB analysis of 25 ug of various whole cell lysates using GTX15780 ERK1 antibody [12D11]. Dilution : 1:1000")
![ICC/IF analysis of A549 cells using GTX15780 ERK1 antibody [12D11]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX15780/GTX15780_311_ICC-IF_w_23060620_404.webp "ICC/IF analysis of A549 cells using GTX15780 ERK1 antibody [12D11]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature")
![ICC/IF analysis of untreated and siRNA knockdown treated HeLa cells using GTX15780 ERK1 antibody [12D11]. Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature Dilution : 1:100 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX15780/GTX15780_312_ICC-IF_w_23060620_855.webp "ICC/IF analysis of untreated and siRNA knockdown treated HeLa cells using GTX15780 ERK1 antibody [12D11]. Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature Dilution : 1:100 for at least 1 hour at room temperature")
![ChIP analysis of 1x10? HCT116 colon carcinoma cells treated with serum for 0, 15, 30, and 60 minutes using GTX15780 ERK1 antibody [12D11]. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to -15kb upstream of the Egr1 gene or exon-1 of Egr1. IP reaction : 1.0ul antibody / 100ul sample](https://www.genetex.com/upload/website/prouct_img/normal/GTX15780/GTX15780_5_ChIP_w_23060620_186.webp "ChIP analysis of 1x10? HCT116 colon carcinoma cells treated with serum for 0, 15, 30, and 60 minutes using GTX15780 ERK1 antibody [12D11]. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to -15kb upstream of the Egr1 gene or exon-1 of Egr1. IP reaction : 1.0ul antibody / 100ul sample")
IHC-P analysis of human colon tissue using GTX15780 ERK1 antibody [12D11]. Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes at 95oC
ERK1 antibody [12D11]
GTX15780
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Primate
TargetMAPK3
Overview
- SupplierGeneTex
- Product NameERK1 antibody [12D11] - KO/KD, Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificityThis antibody does not cross react with ERK2.
- Application Supplier NoteWB: 1:1000. ICC/IF: 2 microg/ml. IHC-P: 1:100. ChIP assay: 1-3 microl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID12D11
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5595
- Target nameMAPK3
- Target descriptionmitogen-activated protein kinase 3
- Target synonymsERK1; ERK-1; ERT2; extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; insulin-stimulated MAP2 kinase; MAPK 1; microtubule-associated protein 2 kinase; mitogen-activated protein kinase 3; P44ERK1; p44-ERK1; P44MAPK; p44-MAPK; PRKM3
- HostMouse
- IsotypeIgG
- Protein IDP27361
- Protein NameMitogen-activated protein kinase 3
- Scientific DescriptionThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Primate
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203