![ELISA analysis of antigen using GTX60492 ERK1 antibody [1E5]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng ELISA analysis of antigen using GTX60492 ERK1 antibody [1E5]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60492/GTX60492_20170912_ELISA_w_23061123_152.webp)
ELISA analysis of antigen using GTX60492 ERK1 antibody [1E5]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng
ERK1 antibody [1E5]
GTX60492
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMAPK3
Overview
- SupplierGeneTex
- Product NameERK1 antibody [1E5]
- Delivery Days Customer9
- Application Supplier NoteWB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- ConjugateUnconjugated
- Gene ID5595
- Target nameMAPK3
- Target descriptionmitogen-activated protein kinase 3
- Target synonymsERK1; ERK-1; ERT2; extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; insulin-stimulated MAP2 kinase; MAPK 1; microtubule-associated protein 2 kinase; mitogen-activated protein kinase 3; P44ERK1; p44-ERK1; P44MAPK; p44-MAPK; PRKM3
- HostMouse
- IsotypeIgG1
- Protein IDP27361
- Protein NameMitogen-activated protein kinase 3
- Scientific DescriptionThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![FACS analysis of HeLa cells using GTX60492 ERK1 antibody [1E5]. Blue : ERK1 Red : negative control FACS analysis of HeLa cells using GTX60492 ERK1 antibody [1E5]. Blue : ERK1 Red : negative control](https://www.genetex.com/upload/website/prouct_img/normal/GTX60492/GTX60492_20170912_FACS_w_23061123_826.webp)
![ICC/IF analysis of NIH3T3 cells using GTX60492 ERK1 antibody [1E5]. Green : ERK1 Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments ICC/IF analysis of NIH3T3 cells using GTX60492 ERK1 antibody [1E5]. Green : ERK1 Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments](https://www.genetex.com/upload/website/prouct_img/normal/GTX60492/GTX60492_20170912_ICCIF_w_23061123_886.webp)
![IHC-P analysis of breast cancer tissue using GTX60492 ERK1 antibody [1E5]. IHC-P analysis of breast cancer tissue using GTX60492 ERK1 antibody [1E5].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60492/GTX60492_20170912_IHC-P_1_w_23061123_976.webp)
![IHC-P analysis of bladder cancer tissue using GTX60492 ERK1 antibody [1E5]. IHC-P analysis of bladder cancer tissue using GTX60492 ERK1 antibody [1E5].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60492/GTX60492_20170912_IHC-P_w_23061123_489.webp)
![WB analysis of human ERK1 (AA: 9-143) recombinant protein using GTX60492 ERK1 antibody [1E5]. WB analysis of human ERK1 (AA: 9-143) recombinant protein using GTX60492 ERK1 antibody [1E5].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60492/GTX60492_20170912_WB_1_w_23061123_123.webp)
![WB analysis of Hela (1), Jurkat (2), RAW264.7 (3), HEK293 (4), K562 (5), NIH3T3 (6), Cos7 (7) and PC-12 (8) cell lysate using GTX60492 ERK1 antibody [1E5]. WB analysis of Hela (1), Jurkat (2), RAW264.7 (3), HEK293 (4), K562 (5), NIH3T3 (6), Cos7 (7) and PC-12 (8) cell lysate using GTX60492 ERK1 antibody [1E5].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60492/GTX60492_20170912_WB_w_23061123_228.webp)




![IHC-P analysis of human colon tissue using GTX15780 ERK1 antibody [12D11]. Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes at 95oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX15780/GTX15780_1040_IHC-P_w_23060620_259.webp)
![WB analysis of various samples using GTX01099 ERK1 antibody [GT1156]. Dilution : 1:1000 Loading : 25 microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX01099/GTX01099_20200508_WB_w_23053121_104.webp)
