ERK1/2 antibody [HL2946] detects ERK1/2 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat esophagus. ERK1/2 stained by ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![ERK1/2 antibody [HL2946] detects ERK1/2 protein by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. ERK1/2 stained by ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX640331/GTX640331_T-45404_20240626_IHC-P_24070822_241.webp "ERK1/2 antibody [HL2946] detects ERK1/2 protein by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. ERK1/2 stained by ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![ERK1/2 antibody [HL2946] detects ERK1/2 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. ERK1/2 stained by ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX640331/GTX640331_T-45404_20240626_IHC-P_M_24070822_459.webp "ERK1/2 antibody [HL2946] detects ERK1/2 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. ERK1/2 stained by ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![ERK1/2 antibody [HL2946] detects ERK1/2 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERK1/2 stained by ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX640331/GTX640331_T-45404_20240524_ICC_IF_24081300_511.webp "ERK1/2 antibody [HL2946] detects ERK1/2 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERK1/2 stained by ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640331/GTX640331_45502_20240830_WB_24090423_173.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640331/GTX640331_45502_20240830_WB_R_24090423_595.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640331/GTX640331_45502_20240830_WB_M_24090423_560.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
ERK1/2 antibody [HL2946] detects ERK1/2 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat esophagus. ERK1/2 stained by ERK1/2 antibody [HL2946] (GTX640331) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ERK1/2 antibody [HL2946]
GTX640331
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAPK3
Overview
- SupplierGeneTex
- Product NameERK1/2 antibody [HL2946]
- Delivery Days Customer7
- Application Supplier NoteIHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2946
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5595
- Target nameMAPK3
- Target descriptionmitogen-activated protein kinase 3
- Target synonymsERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK, mitogen-activated protein kinase 3, MAPK 1, extracellular signal-regulated kinase 1, extracellular signal-related kinase 1, insulin-stimulated MAP2 kinase, microtubule-associated protein 2 kinase
- HostRabbit
- IsotypeIgG
- Protein IDP27361
- Protein NameMitogen-activated protein kinase 3
- Scientific DescriptionThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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