Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) SKOV3 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab MAPK-12D4 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) SKOV3 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab MAPK-12D4 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
ERK1 (MAPK3) (pT-E-pY Motif) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 12D4]
AM00084PU-N
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetMAPK3
Overview
- SupplierOriGene
- Product NameERK1 (MAPK3) (pT-E-pY Motif) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 12D4]
- Delivery Days Customer14
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID12D4
- Gene ID5595
- Target nameMAPK3
- Target descriptionmitogen-activated protein kinase 3
- Target synonymsERK1; ERK-1; ERT2; extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; insulin-stimulated MAP2 kinase; MAPK 1; microtubule-associated protein 2 kinase; mitogen-activated protein kinase 3; P44ERK1; p44-ERK1; P44MAPK; p44-MAPK; PRKM3
- HostMouse
- IsotypeIgG1
- Scientific DescriptionERK1 (MAPK3) (pT-E-pY Motif) (incl. pos. control) mouse monoclonal antibody, clone 12D4, Purified
- ReactivityCanine, Human, Mouse, Rat
- UNSPSC12352203