ProductsBack/ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173]

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:500 and competitor's antibody (# Highly Cited Antibody) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.

ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173]

GTX635617

ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Product group Antibodies

ReactivityDrosophila, Human, Mouse, Rat

TargetMAPK3

Overview

Supplier
GeneTex
Product Name
ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173]
Delivery Days Customer
9
Application Supplier Note
WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Applications
ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Certification
Research Use Only
Clonality
Monoclonal
Clone ID
HL173
Concentration
1 mg/ml
Conjugate
Unconjugated
Gene ID5595
Target name
MAPK3
Target description
mitogen-activated protein kinase 3
Target synonyms
ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK, mitogen-activated protein kinase 3, MAPK 1, extracellular signal-regulated kinase 1, extracellular signal-related kinase 1, insulin-stimulated MAP2 kinase, microtubule-associated protein 2 kinase
Host
Rabbit
Isotype
IgG
Protein IDP27361
Protein Name
Mitogen-activated protein kinase 3
Scientific Description
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
Reactivity
Drosophila, Human, Mouse, Rat
Storage Instruction
-20°C or -80°C,2°C to 8°C
UNSPSC
12352203

References

Watanabe M, Kawaguchi K, Nakamura Y, et al. GIF-2209, an Oxindole Derivative, Accelerates Melanogenesis and Melanosome Secretion via the Modification of Lysosomes in B16F10 Mouse Melanoma Cells. Molecules. 2021,27(1). doi: 10.3390/molecules27010177
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Chung WP, Huang WL, Liao WA, et al. FTY720 in resistant human epidermal growth factor receptor 2-positive breast cancer. Sci Rep. 2022,12(1):241. doi: 10.1038/s41598-021-04328-y
Read this paper
Tsai HJ, Shih CC, Chang KY, et al. Angiotensin-(1-7) treatment blocks lipopolysaccharide-induced organ damage, platelet dysfunction, and IL-6 and nitric oxide production in rats. Sci Rep. 2021,11(1):610. doi: 10.1038/s41598-020-79902-x
Read this paper
Liu JF, Chi MC, Lin CY, et al. PM2.5 facilitates IL-6 production in human osteoarthritis synovial fibroblasts via ASK1 activation. J Cell Physiol. 2021,236(3):2205-2213. doi: 10.1002/jcp.30009
Read this paper