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ERP29 Monoclonal Antibody

BSM-60396M
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetERP29
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    ERP29 Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(), ICC/IF(1:50-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    H8F11
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10961
  • Target name
    ERP29
  • Target description
    endoplasmic reticulum protein 29
  • Target synonyms
    C12orf8; endoplasmic reticulum lumenal protein ERp28; endoplasmic reticulum resident protein 28; endoplasmic reticulum resident protein 29; endoplasmic reticulum resident protein 31; epididymis secretory protein Li 107; ERp28; ERp31; HEL-S-107; PDIA9; PDI-DB; protein disulfide isomerase family A, member 9
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP30040
  • Protein Name
    Endoplasmic reticulum resident protein 29
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western blot All lanes: ERP29 antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 29, 6 kDa Observed band size: 80 kDa
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Figure 1. Western blot analysis of ERP29 using anti-ERP29 antibody (A03621-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human placenta tissue lysates, Lane 7: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERP29 antigen affinity purified polyclonal antibody (Catalog # A03621-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERP29 at approximately 29 kDa. The expected band size for ERP29 is at 29 kDa.
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