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Goat anti-ERP29

EB08977
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetERP29
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ERP29 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence:This product has been successfully used in IF on Rat: Gao J et al, Mol Med Rep. 2016 Jan;13(1):398-402. PMID: 26548430.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID10961
  • Target name
    ERP29
  • Target description
    endoplasmic reticulum protein 29
  • Target synonyms
    C12orf8, ERp28, ERp31, HEL-S-107, PDI-DB, PDIA9, endoplasmic reticulum resident protein 29, endoplasmic reticulum lumenal protein ERp28, endoplasmic reticulum resident protein 28, endoplasmic reticulum resident protein 31, epididymis secretory protein Li 107, protein disulfide isomerase family A, member 9
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_006808.1. GeneIDs all Nonhuman: 67397 (mouse); 117030 (rat);. Purification: Antigen affinity purified. Names and symbols: ERP29; endoplasmic reticulum protein 29; C12orf8; ERp28; ERp31; PDI-DB; endoplasmic reticulum lumenal protein ERp28
  • Reactivity Supplier
    Human, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Gao J, Zhang Y, Wang L, et al. Endoplasmic reticulum protein 29 is involved in endoplasmic reticulum stress in islet beta cells. Mol Med Rep. 2016,13(1):398-402. doi: 10.3892/mmr.2015.4527
    Read this paper

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Figure 1. Western blot analysis of ERP29 using anti-ERP29 antibody (A03621-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human placenta tissue lysates, Lane 7: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERP29 antigen affinity purified polyclonal antibody (Catalog # A03621-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERP29 at approximately 29 kDa. The expected band size for ERP29 is at 29 kDa.
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