Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERRFI1 Polyclonal Antibody, Unconjugated (bs-14630R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERRFI1 Polyclonal Antibody, Unconjugated (bs-14630R) at 1:400 overnight at 4°C, DAB staining.
ERRFI1 Polyclonal Antibody
BS-14630R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Rat, Sheep
TargetERRFI1
Overview
- SupplierBioss
- Product NameERRFI1 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID54206
- Target nameERRFI1
- Target descriptionERBB receptor feedback inhibitor 1
- Target synonymsGENE-33, MIG-6, MIG6, RALT, ERBB receptor feedback inhibitor 1, mitogen-inducible gene 6 protein, receptor-associated late transducer
- HostRabbit
- IsotypeIgG
- Protein IDQ9UJM3
- Protein NameERBB receptor feedback inhibitor 1
- ReactivityBovine, Canine, Human, Mouse, Rat, Sheep
- Storage Instruction-20°C
- UNSPSC41116161
Datasheet
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![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Mig-6 antibody [N1C1] (GTX118238) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX118238/GTX118238_44650_20220422_WB_23011618_596.webp)
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