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Cross-reactivity assessment of ESR1 Mouse Monoclonal Antibody AE00272 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
Cross-reactivity assessment of ESR1 Mouse Monoclonal Antibody AE00272 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
Cross-reactivity assessment of ESR1 Mouse Monoclonal Antibody AE00272 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.

ESR1 Monoclonal Antibody AE00272

AE00272
Aeonian Biotech
TargetESR1
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Product group Antibodies
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Overview

  • Supplier
    Aeonian Biotech
  • Product Name
    ESR1 Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    IHC, PA, WB
  • Category Supplier
    Antibody
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ESR1/1904
  • Conjugate
    Unconjugated
  • Gene ID2099
  • Target name
    ESR1
  • Target description
    estrogen receptor 1
  • Target synonyms
    E2 receptor alpha; ER; Era; ER-alpha; ESR; ESRA; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; ESTRR; NR3A1; nuclear receptor subfamily 3 group A member 1; oestrogen receptor alpha
  • Host
    Mouse
  • Isotype
    IgG2a kappa
  • Protein IDP03372
  • Protein Name
    Estrogen receptor
  • Scientific Description
    ESR1 Monoclonal Antibody AE00272
  • Shelf life instruction
    Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
  • Reactivity Supplier
    Human
  • Storage Instruction
    2-8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Estrogen Receptor using anti-Estrogen Receptor antibody (PB9192). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Estrogen Receptor antigen affinity purified polyclonal antibody (Catalog # PB9192) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Estrogen Receptor at approximately 66 kDa. The expected band size for Estrogen Receptor is at 66 kDa.
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