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ProductsBack/ESR1/ER (5C1) Monoclonal Antibody
Lane 1: MCF-7 Cells; 35ug of protein lysate loaded; Probed ESR1/ER (5C1) Monoclonal Antibody, unconjugated (bsm-51124M) at 1:200 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: MCF-7 Cells; 35ug of protein lysate loaded; Probed ESR1/ER (5C1) Monoclonal Antibody, unconjugated (bsm-51124M) at 1:200 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: MCF-7 Cells; 35ug of protein lysate loaded; Probed ESR1/ER (5C1) Monoclonal Antibody, unconjugated (bsm-51124M) at 1:200 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

ESR1/ER (5C1) Monoclonal Antibody

BSM-51124M
Bioss
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetESR1
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Overview

  • Supplier
    Bioss
  • Product Name
    ESR1/ER (5C1) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(1:300-5000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5C1
  • Concentration
    0.5 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID2099
  • Target name
    ESR1
  • Target description
    estrogen receptor 1
  • Target synonyms
    ER, ESR, ESRA, ESTRR, Era, NR3A1, estrogen receptor, E2 receptor alpha, ER-alpha, estradiol receptor, estrogen nuclear receptor alpha, estrogen receptor alpha E1-E2-1-2, estrogen receptor alpha E1-N2-E2-1-2, nuclear receptor subfamily 3 group A member 1, oestrogen receptor alpha
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP03372
  • Protein Name
    Estrogen receptor
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Estrogen Receptor using anti-Estrogen Receptor antibody (PB9192). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Estrogen Receptor antigen affinity purified polyclonal antibody (Catalog # PB9192) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Estrogen Receptor at approximately 66 kDa. The expected band size for Estrogen Receptor is at 66 kDa.
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