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ProductsBack/ESR2 / ER Beta Antibody (N-Terminus)

ESR2 / ER Beta Antibody (N-Terminus)

LS-C171919
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetESR2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ESR2 / ER Beta Antibody (N-Terminus)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (0.5 - 1 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2100
  • Target name
    ESR2
  • Target description
    estrogen receptor 2
  • Target synonyms
    Erb; ER-BETA; ESRB; ESR-BETA; ESTRB; estrogen receptor beta; estrogen receptor beta 4; estrogen receptor beta splice variant, ERbeta2delta7; estrogen receptor beta splice variant, ERbeta4delta7; estrogen receptor beta splice variant, ERbeta6; estrogen receptor beta splice variant, ERbeta6delta7; estrogen receptor beta splice variant, ERbeta7; estrogen receptor beta splice variant, ERbeta7delta7; estrogen receptor beta splice variant, ERbetaEx. 4L; estrogen receptor beta splice variant, ERbetaEx. 6L; NR3A2; nuclear receptor subfamily 3 group A member 2; ODG8; oestrogen receptor beta
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ESR2 using anti-ESR2 antibody (A00786-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESR2 antigen affinity purified polyclonal antibody (Catalog # A00786-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESR2 at approximately 59 kDa. The expected band size for ESR2 is at 59 kDa.
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