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Estrogen Receptor(ER505), Biotin conjugate, 0.1mg/mL [26628-22-8]

Estrogen Receptor(ER505), Biotin conjugate, 0.1mg/mL [26628-22-8]

BNCB0505
Biotium
ApplicationsWestern Blot
Product group Antibodies
TargetESR1
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Overview

  • Supplier
    Biotium
  • Product Name
    Estrogen Receptor(ER505), Biotin conjugate, 0.1mg/mL
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ER505
  • Concentration
    0.1 mg/ml
  • Conjugate
    Biotin
  • Gene ID2099
  • Target name
    ESR1
  • Target description
    estrogen receptor 1
  • Target synonyms
    E2 receptor alpha; ER; Era; ER-alpha; ESR; ESRA; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; ESTRR; NR3A1; nuclear receptor subfamily 3 group A member 1; oestrogen receptor alpha
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP03372
  • Protein Name
    Estrogen receptor
  • Scientific Description
    This MAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. This MAb is excellent for staining of formalin-fixed, paraffin-embedded breast carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
  • Source
    Animal
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Estrogen Receptor using anti-Estrogen Receptor antibody (PB9192). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Estrogen Receptor antigen affinity purified polyclonal antibody (Catalog # PB9192) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Estrogen Receptor at approximately 66 kDa. The expected band size for Estrogen Receptor is at 66 kDa.
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