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EWSR1 antibody detects EWSR1 protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. EWSR1 antibody (GTX114069) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
EWSR1 antibody detects EWSR1 protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. EWSR1 antibody (GTX114069) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
EWSR1 antibody detects EWSR1 protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. EWSR1 antibody (GTX114069) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min

EWSR1 antibody

GTX114069
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetEWSR1
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Overview

  • Supplier
    GeneTex
  • Product Name
    EWSR1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.18 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2130
  • Target name
    EWSR1
  • Target description
    EWS RNA binding protein 1
  • Target synonyms
    EWS, EWS-FLI1, RNA-binding protein EWS, Ewing sarcoma breakpoint region 1, Ewings sarcoma EWS-Fli1 (type 1) oncogene
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01844
  • Protein Name
    RNA-binding protein EWS
  • Scientific Description
    This gene encodes a putative RNA binding protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in two products. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EWSR1 using anti-EWSR1 antibody (PB9585). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EWSR1 antigen affinity purified polyclonal antibody (Catalog # PB9585) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EWSR1 at approximately 95 kDa. The expected band size for EWSR1 is at 68 kDa.
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Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EWSR1 antibody [HL3150] (GTX640660) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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