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Western Blot analysis of COLO205 cells using EWS Polyclonal Antibody
Western Blot analysis of COLO205 cells using EWS Polyclonal Antibody
Western Blot analysis of COLO205 cells using EWS Polyclonal Antibody

EWSR1 Antibody

CSB-PA002452
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetEWSR1
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Overview

  • Supplier
    Cusabio
  • Product Name
    EWSR1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2130
  • Target name
    EWSR1
  • Target description
    EWS RNA binding protein 1
  • Target synonyms
    bK984G1.4; Ewing sarcoma breakpoint region 1; Ewings sarcoma EWS-Fli1 (type 1) oncogene; EWS; EWS RNA-binding protein variant 6; EWS-FLI1; RNA-binding protein EWS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01844
  • Protein Name
    RNA-binding protein EWS
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EWSR1 using anti-EWSR1 antibody (PB9585). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EWSR1 antigen affinity purified polyclonal antibody (Catalog # PB9585) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EWSR1 at approximately 95 kDa. The expected band size for EWSR1 is at 68 kDa.
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