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Western Blot analysis of A549 cells using Exo1 Polyclonal Antibody
Western Blot analysis of A549 cells using Exo1 Polyclonal Antibody
Western Blot analysis of A549 cells using Exo1 Polyclonal Antibody

EXO1 Antibody

CSB-PA002455
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetEXO1
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Overview

  • Supplier
    Cusabio
  • Product Name
    EXO1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9156
  • Target name
    EXO1
  • Target description
    exonuclease 1
  • Target synonyms
    HEX1, hExoI, exonuclease 1, rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UQ84
  • Protein Name
    Exonuclease 1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Exonuclease 1/EXO1 using anti-Exonuclease 1/EXO1 antibody (A00536-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Exonuclease 1/EXO1 antigen affinity purified polyclonal antibody (Catalog # A00536-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Exonuclease 1/EXO1 at approximately 120 kDa. The expected band size for Exonuclease 1/EXO1 is at 94 kDa.
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