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Exonuclease 1 antibody

GTX109891
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetEXO1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Exonuclease 1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9156
  • Target name
    EXO1
  • Target description
    exonuclease 1
  • Target synonyms
    HEX1, hExoI, exonuclease 1, rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UQ84
  • Protein Name
    Exonuclease 1
  • Scientific Description
    This gene encodes a protein with 5 to 3 exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Xiao H, Li F, Mladenov E, et al. Increased Resection at DSBs in G(2)-Phase Is a Unique Phenotype Associated with DNA-PKcs Defects That Is Not Shared by Other Factors of c-NHEJ. Cells. 2022,11(13). doi: 10.3390/cells11132099
    Read this paper
  • Elbæk CR, Petrosius V, Benada J, et al. WEE1 kinase protects the stability of stalled DNA replication forks by limiting CDK2 activity. Cell Rep. 2022,38(3):110261. doi: 10.1016/j.celrep.2021.110261
    Read this paper
  • Rall-Scharpf M, Friedl TWP, Biechonski S, et al. Sex-specific differences in DNA double-strand break repair of cycling human lymphocytes during aging. Aging (Albany NY). 2021,13(17):21066-21089. doi: 10.18632/aging.203519
    Read this paper
  • Kim JJ, Lee SY, Choi JH, et al. PCAF-Mediated Histone Acetylation Promotes Replication Fork Degradation by MRE11 and EXO1 in BRCA-Deficient Cells. Mol Cell. 2020,80(2):327-344.e8. doi: 10.1016/j.molcel.2020.08.018
    Read this paper
  • Volcic M, Sparrer KMJ, Koepke L, et al. Vpu modulates DNA repair to suppress innate sensing and hyper-integration of HIV-1. Nat Microbiol. 2020,5(10):1247-1261. doi: 10.1038/s41564-020-0753-6
    Read this paper
  • Burdova K, Yang H, Faedda R, et al. E2F1 proteolysis via SCF-cyclin F underlies synthetic lethality between cyclin F loss and Chk1 inhibition. EMBO J. 2019,38(20):e101443. doi: 10.15252/embj.2018101443
    Read this paper
  • Chen YJ, Tsai CH, Wang PY, et al. SMYD3 Promotes Homologous Recombination via Regulation of H3K4-mediated Gene Expression. Sci Rep. 2017,7(1):3842. doi: 10.1038/s41598-017-03385-6
    Read this paper
  • Hampp S, Kiessling T, Buechle K, et al. DNA damage tolerance pathway involving DNA polymerase ι and the tumor suppressor p53 regulates DNA replication fork progression. Proc Natl Acad Sci U S A. 2016,113(30):E4311-9. doi: 10.1073/pnas.1605828113
    Read this paper
  • Zhou Y, Caron P, Legube G, et al. Quantitation of DNA double-strand break resection intermediates in human cells. Nucleic Acids Res. 2014,42(3):e19. doi: 10.1093/nar/gkt1309
    Read this paper
  • Zhou Y, Paull TT. DNA-dependent protein kinase regulates DNA end resection in concert with Mre11-Rad50-Nbs1 (MRN) and ataxia telangiectasia-mutated (ATM). J Biol Chem. 2013,288(52):37112-25. doi: 10.1074/jbc.M113.514398
    Read this paper

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Figure 1. Western blot analysis of Exonuclease 1/EXO1 using anti-Exonuclease 1/EXO1 antibody (A00536-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Exonuclease 1/EXO1 antigen affinity purified polyclonal antibody (Catalog # A00536-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Exonuclease 1/EXO1 at approximately 120 kDa. The expected band size for Exonuclease 1/EXO1 is at 94 kDa.
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