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EYA4 antibody

GTX131408
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetEYA4
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Overview

  • Supplier
    GeneTex
  • Product Name
    EYA4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.26 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2070
  • Target name
    EYA4
  • Target description
    EYA transcriptional coactivator and phosphatase 4
  • Target synonyms
    CMD1J; DFNA10; dJ78N10.1 (eyes absent); eyes absent homolog 4; eyes absent-like protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95677
  • Protein Name
    Eyes absent homolog 4
  • Scientific Description
    This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator through its protein phosphatase activity, and it may be important for eye development, and for continued function of the mature organ of Corti. Mutations in this gene are associated with postlingual, progressive, autosomal dominant hearing loss at the deafness, autosomal dominant nonsyndromic sensorineural 10 locus. Defects in this gene are also associated with dilated cardiomyopathy 1J. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EYA4 using anti-EYA4 antibody (A04516-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: monkey skeletal muscle tissue lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: rat skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EYA4 antigen affinity purified polyclonal antibody (Catalog # A04516-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EYA4 at approximately 50KD. The expected band size for EYA4 is at 50KD.
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