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ProductsBack/F2 / Prothrombin / Thrombin Antibody (clone 318, Preservative Free)

F2 / Prothrombin / Thrombin Antibody (clone 318, Preservative Free)

LS-C819282
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetF2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    F2 / Prothrombin / Thrombin Antibody (clone 318, Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    318
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2147
  • Target name
    F2
  • Target description
    coagulation factor II, thrombin
  • Target synonyms
    PT, RPRGL2, THPH1, prothrombin, prepro-coagulation factor II, prothrombin B-chain, thrombin factor II
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Prothrombin using anti-Prothrombin antibody (A00044). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human plasma lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prothrombin antigen affinity purified polyclonal antibody (Catalog # A00044) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prothrombin at approximately 85KD. The expected band size for Prothrombin is at 70KD.
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