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ProductsBack/F4/80 antibody [CI:A3-1]
IHC-Fr analysis of mouse spleen tissue using GTX26640 F4/80 antibody [CI:A3-1].
IHC-Fr analysis of mouse spleen tissue using GTX26640 F4/80 antibody [CI:A3-1].
IHC-Fr analysis of mouse spleen tissue using GTX26640 F4/80 antibody [CI:A3-1].

F4/80 antibody [CI:A3-1]

GTX26640
GeneTex
ApplicationsElectron Microscopy, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, RadioImmunoAssay
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAdgre1
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Overview

  • Supplier
    GeneTex
  • Product Name
    F4/80 antibody [CI:A3-1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    FCM: 1/50-1/100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Electron Microscopy, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, RadioImmunoAssay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CI:A3-1
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID13733
  • Target name
    Adgre1
  • Target description
    adhesion G protein-coupled receptor E1
  • Target synonyms
    DD7A5-7, EGF-TM7, Emr1, F4/80, Gpf480, Ly71, TM7LN3, adhesion G protein-coupled receptor E1, EGF-like module containing, mucin-like, hormone receptor-like sequence 1, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor, cell surface glycoprotein F4/80
  • Host
    Rat
  • Isotype
    IgG2b
  • Protein IDQ61549
  • Protein Name
    Adhesion G protein-coupled receptor E1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

References

  • The potential role of hydrogen sulfide in regulating macrophage phenotypic changes via PINK1/parkin-mediated mitophagy in sepsis-related cardiorenal syndrome.
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  • Adventitial macrophage accumulation impairs perivascular nerve function in mesenteric arteries with inflammatory bowel disease.
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  • Sestrin2 Is Increased in Calcific Aortic Disease and Inhibits Osteoblastic Differentiation in Valvular Interstitial Cells via the Nuclear Factor E2-related Factor 2 Pathway.
    Read this paper
  • Targeting prooxidant MnSOD effect inhibits triple-negative breast cancer (TNBC) progression and M2 macrophage functions under the oncogenic stress. Al Haq AT et al., 2022 Jan 11, Cell Death Dis
    Read this paper
  • Lupeol reduces M1 macrophage polarization to attenuate immunologic dissonance and fatty acid deposition in rats with diet-induced metabolic syndrome. Li J et al., 2021 Oct, Ann Transl Med
    Read this paper

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Figure 1. IHC analysis of F4/80/Adgre1 using anti-F4/80/Adgre1 antibody (A08751). F4/80/Adgre1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-F4/80/Adgre1 Antibody (A08751) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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FACS analysis of mouse C57Bl/6 bone marrow cells using GTX01470-02 F4/80 antibody [BM8.1] (Biotin). Right panel : co-stained with F4/80 antibody [BM8.1] (Biotin) and Mouse CD11b antibody (APC) Left panel : co-stained with isotype control and Mouse CD11b antibody (APC) antibody amount : 0.125 μg (5 μl)
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FACS analysis of mouse C57Bl/6 bone marrow cells using GTX01470-08 F4/80 antibody [BM8.1] (PE). Right panel : co-stained with F4/80 antibody [BM8.1] (PE) and Mouse CD11b antibody (APC) Left panel : co-stained with isotype control and Mouse CD11b antibody (APC) antibody amount : 0.25 μg (5 μl)
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