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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA007938LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA007938LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA007938LA01HU at dilution of 1:100

FAAH Antibody

CSB-PA007938LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFAAH
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Overview

  • Supplier
    Cusabio
  • Product Name
    FAAH Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2166
  • Target name
    FAAH
  • Target description
    fatty acid amide hydrolase
  • Target synonyms
    FAAH-1, FAAH1, PSAB, fatty-acid amide hydrolase 1, anandamide amidohydrolase 1, fatty acid ester hydrolase, oleamide hydrolase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00519
  • Protein Name
    Fatty-acid amide hydrolase 1
  • Scientific Description
    Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FAAH1/FAAH using anti-FAAH1/FAAH antibody (A00801-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAAH1/FAAH antigen affinity purified polyclonal antibody (Catalog # A00801-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAAH1/FAAH at approximately 63 kDa. The expected band size for FAAH1/FAAH is at 63 kDa.
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