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I-FABP Antibody (YA2788, Rabbit)

HY-P83043
MedChem Express
TargetFABP2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    I-FABP Antibody (YA2788, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2169
  • Target name
    FABP2
  • Target description
    fatty acid binding protein 2
  • Target synonyms
    FABPI; fatty acid binding protein 2, intestinal; fatty acid-binding protein, intestinal; I-FABP; intestinal-type fatty acid-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    I-FABP Antibody (YA2788) is a non-conjugated IgG antibody, targeting FABP2, with a predicted molecular weight of 15 kDa (observed band size: 15 kDa). I-FABP Antibody (YA2788) can be used for WB experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FABP2/I-FABP using anti-FABP2/I-FABP antibody (PB9943). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP2/I-FABP antigen affinity purified polyclonal antibody (Catalog # PB9943) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP2/I-FABP at approximately 15 kDa. The expected band size for FABP2/I-FABP is at 15 kDa.
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