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Western Blot Positive WB detected in: 293T whole cell lysate, U251 whole cell lysate, PC-3 whole cell lysate, Hela whole cell lysate, U87 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate All lanes: FABP4 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 kDa Observed band size: 15 kDa
Western Blot Positive WB detected in: 293T whole cell lysate, U251 whole cell lysate, PC-3 whole cell lysate, Hela whole cell lysate, U87 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate All lanes: FABP4 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 kDa Observed band size: 15 kDa
Western Blot Positive WB detected in: 293T whole cell lysate, U251 whole cell lysate, PC-3 whole cell lysate, Hela whole cell lysate, U87 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate All lanes: FABP4 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 kDa Observed band size: 15 kDa

FABP4 Antibody

CSB-PA007945LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFABP4
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Overview

  • Supplier
    Cusabio
  • Product Name
    FABP4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2167
  • Target name
    FABP4
  • Target description
    fatty acid binding protein 4
  • Target synonyms
    A-FABP, AFABP, ALBP, HEL-S-104, aP2, fatty acid-binding protein, adipocyte, adipocyte fatty acid binding protein, adipocyte lipid-binding protein, adipocyte-type fatty acid-binding protein, epididymis secretory protein Li 104, fatty acid binding protein 4, adipocyte
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15090
  • Protein Name
    Fatty acid-binding protein, adipocyte
  • Scientific Description
    Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of FABP4 using anti-FABP4 antibody (RP1085). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP4 antigen affinity purified polyclonal antibody (Catalog # RP1085) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP4 at approximately 15 kDa. The expected band size for FABP4 is at 15 kDa.
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