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Western blot analysis using anti-FABP4 (human), pAb (Prod. No. AG-25A-0041) at 1:2000 dilution. 1: Human FABP4 (His-tagged).
Western blot analysis using anti-FABP4 (human), pAb (Prod. No. AG-25A-0041) at 1:2000 dilution. 1: Human FABP4 (His-tagged).
Western blot analysis using anti-FABP4 (human), pAb (Prod. No. AG-25A-0041) at 1:2000 dilution. 1: Human FABP4 (His-tagged).

anti-FABP4 (human), pAb

AG-25A-0041
AdipoGen Life Sciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetFABP4
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Overview

  • Supplier
    AdipoGen Life Sciences
  • Product Name
    anti-FABP4 (human), pAb
  • Delivery Days Customer
    10
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID2167
  • Target name
    FABP4
  • Target description
    fatty acid binding protein 4
  • Target synonyms
    adipocyte fatty acid binding protein; adipocyte lipid-binding protein; adipocyte-type fatty acid-binding protein; AFABP; A-FABP; ALBP; aP2; epididymis secretory protein Li 104; fatty acid binding protein 4, adipocyte; fatty acid-binding protein, adipocyte; HEL-S-104
  • Host
    Rabbit
  • Protein IDP15090
  • Protein Name
    Fatty acid-binding protein, adipocyte
  • Scientific Description
    FABP4 is a fatty acid binding protein in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs regulate the fatty acid uptake, transport, and metabolism. aP2, the mouse form of FABP4, seems to be central to the pathway that links obesity to insulin resistance. aP2 was shown to be an adipokine linking adipocytes to hepatic glucose production and that neutralizing secreted aP2 may represent an effective therapeutic strategy against diabetes. - Polyclonal Antibody. Recognizes human FABP4. Detects a band of ~15kDa by Western blot. Source: Rabbit. Applications: ELISA, WB. Liquid. 0.2microm-filtered solution in PBS, pH 7.4. Contains no preservatives. FABP4 is a fatty acid binding protein in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs regulate the fatty acid uptake, transport, and metabolism. aP2, the mouse form of FABP4, seems to be central to the pathway that links obesity to insulin resistance. aP2 was shown to be an adipokine linking adipocytes to hepatic glucose production and that neutralizing secreted aP2 may represent an effective therapeutic strategy against diabetes.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FABP4 using anti-FABP4 antibody (RP1085). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP4 antigen affinity purified polyclonal antibody (Catalog # RP1085) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP4 at approximately 15 kDa. The expected band size for FABP4 is at 15 kDa.
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