FACL4 antibody [HL44] detects FACL4 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. FACL4 stained by FACL4 antibody [HL44] (GTX635616) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Drosophila tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL44] (GTX635616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635616/GTX635616_44020_20230331_WB_Drosophila_brain_23041023_346.webp "Drosophila tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL44] (GTX635616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.")
![Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL44] (GTX635616) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635616/GTX635616_44020_20200918_WB_shRNA_watermark_w_23061202_763.webp "Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL44] (GTX635616) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL229] (GTX635616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635616/GTX635616_44020_20210611_WB_R_w_23061202_419.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL229] (GTX635616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL229] (GTX635616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635616/GTX635616_44020_20210611_WB_M_w_23061202_519.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL229] (GTX635616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![FACL4 antibody [HL229] detects FACL4 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FACL4 stained by FACL4 antibody [HL229] (GTX635616) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635616/GTX635616_44020_20200729_ICC_IF_w_23061202_960.webp "FACL4 antibody [HL229] detects FACL4 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FACL4 stained by FACL4 antibody [HL229] (GTX635616) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10 μm.")
![FACL4 antibody [HL229] detects FACL4 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. FACL4 stained by FACL4 antibody [HL229] (GTX635616) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX635616/GTX635616_44020_20200814_IHC-P_M_w_23061202_525.webp "FACL4 antibody [HL229] detects FACL4 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. FACL4 stained by FACL4 antibody [HL229] (GTX635616) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL229] (GTX635616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635616/GTX635616_44907_20221230_WB_TPM_watermark_23073119_693.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL229] (GTX635616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
FACL4 antibody [HL44] detects FACL4 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. FACL4 stained by FACL4 antibody [HL44] (GTX635616) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
FACL4 antibody [HL229]
GTX635616
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityDrosophila, Human, Mouse, Rat
TargetACSL4
Overview
- SupplierGeneTex
- Product NameFACL4 antibody [HL229]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL229
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2182
- Target nameACSL4
- Target descriptionacyl-CoA synthetase long chain family member 4
- Target synonymsACS4, FACL4, LACS4, MRX63, MRX68, XLID63, long-chain-fatty-acid--CoA ligase 4, acyl-CoA synthetase 4, arachidonate--CoA ligase, fatty-acid-Coenzyme A ligase, long-chain 4, lignoceroyl-CoA synthase, long-chain acyl-CoA synthetase 4, long-chain fatty-acid-Coenzyme A ligase 4
- HostRabbit
- IsotypeIgG
- Protein IDO60488
- Protein NameLong-chain-fatty-acid--CoA ligase 4
- Scientific DescriptionThe protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants. [provided by RefSeq, Jan 2016]
- ReactivityDrosophila, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX114399/GTX114399_40184_20170508_IHC-P_R_w_23060501_459.webp)
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