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ProductsBack/Factor VIII Polyclonal Antibody, APC Conjugated

Factor VIII Polyclonal Antibody, APC Conjugated

BS-2974R-APC
Bioss Antibodies
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetF8
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Factor VIII Polyclonal Antibody, APC Conjugated
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    APC (Allophycocyanin)
  • Gene ID2157
  • Target name
    F8
  • Target description
    coagulation factor VIII
  • Target synonyms
    AHF; antihemophilic factor; coagulation factor VIII; coagulation factor VIII A1 domain; coagulation factor VIII C2 domain; coagulation factor VIII, procoagulant component; coagulation factor VIIIc; DXS1253E; F8B; F8C; factor VIII F8B; FVIII; HEMA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00451
  • Protein Name
    Coagulation factor VIII
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Factor VIII using anti-Factor VIII antibody (PA2061). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: human Hela whole cell lysates, Lane 7: human HepG2 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor VIII antigen affinity purified polyclonal antibody (Catalog # PA2061) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Factor VIII at approximately 92KD. The expected band size for Factor VIII is at 92KD.
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