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Western Blot analysis of K562 cells using FAF1 Polyclonal Antibody
Western Blot analysis of K562 cells using FAF1 Polyclonal Antibody
Western Blot analysis of K562 cells using FAF1 Polyclonal Antibody

FAF1 Antibody

CSB-PA004921
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetFAF1
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Overview

  • Supplier
    Cusabio
  • Product Name
    FAF1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11124
  • Target name
    FAF1
  • Target description
    Fas associated factor 1
  • Target synonyms
    CGI-03; Fas (TNFRSF6) associated factor 1; FAS-associated factor 1; hFAF1; HFAF1s; TNFRSF6-associated factor 1; UBX domain protein 3A; UBX domain-containing protein 12; UBX domain-containing protein 3A; UBXD12; UBXN3A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UNN5
  • Protein Name
    FAS-associated factor 1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FAF1 using anti-FAF1 antibody (A03842-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAF1 antigen affinity purified polyclonal antibody (Catalog # A03842-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAF1 at approximately 74 kDa. The expected band size for FAF1 is at 74 kDa.
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