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Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA875683LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA875683LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA875683LA01HU at dilution of 1:100

FAM134B Antibody

CSB-PA875683LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRETREG1
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Overview

  • Supplier
    Cusabio
  • Product Name
    FAM134B Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54463
  • Target name
    RETREG1
  • Target description
    reticulophagy regulator 1
  • Target synonyms
    FAM134B, JK-1, JK1, reticulophagy regulator 1, family with sequence similarity 134 member B, protein FAM134B, reticulophagy receptor 1, reticulophagy receptor FAM134B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H6L5
  • Protein Name
    Reticulophagy regulator 1
  • Scientific Description
    Endoplasmic reticulum-anchored autophagy receptor that mediates ER delivery into lysosomes through sequestration into autophagosomes (PubMed:26040720). Promotes membrane remodeling and ER scission via its membrane bending capacity and targets the fragments into autophagosomes via interaction with ATG8 family proteins (PubMed:26040720). Required for long-term survival of nociceptive and autonomic ganglion neurons (PubMed:19838196, PubMed:26040720).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RETREG1 using anti-RETREG1 antibody (A32125). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RETREG1 antigen affinity purified polyclonal antibody (Catalog # A32125) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RETREG1 at approximately 55 kDa. The expected band size for RETREG1 is at 55 kDa.
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