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ProductsBack/Fas Associating Death Domain Containing Protein (FADD) Polyclonal Antibody

Fas Associating Death Domain Containing Protein (FADD) Polyclonal Antibody

CAU21614
Biomatik
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetFadd
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Overview

  • Supplier
    Biomatik
  • Product Name
    Fas Associating Death Domain Containing Protein (FADD) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID14082
  • Target name
    Fadd
  • Target description
    Fas associated via death domain
  • Target synonyms
    Mort1/FADD, FAS-associated death domain protein, FAS-associating death domain-containing protein, Fas (TNFRSF6)-associated via death domain, Fas-associating protein with death domain, mediator of receptor induced toxicity, protein FADD
  • Host
    Rabbit
  • Protein IDQ61160
  • Protein Name
    FAS-associated death domain protein
  • Scientific Description
    The Fas Associating Death Domain Containing Protein (FADD) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC.
  • Reactivity
    Mouse, Rat
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FADD using anti-FADD antibody (A00237-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates, Lane 2: mouse stomach tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FADD antigen affinity purified polyclonal antibody (Catalog # A00237-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FADD at approximately 30KD. The expected band size for FADD is at 23KD.
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Western blot analysis of extracts from A549 cells (Lane 2), using FADD (Ab-191) antiobdy. The lane on the left is treated with synthesized peptide.
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Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with FADD Polyclonal Antibody (bs-0511R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
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